Different distributions of interstitial cells of Cajal and platelet-derived growth factor receptor-α positive cells in colonic smooth muscle cell/interstitial cell of Cajal/platelet-derived growth factor receptor-α positive cell syncytium in mice

Figure 2 Effects of L-NAME and NPPB on smooth muscle contractions of the colon. A and C: Increased effects of L-NAME on colonic migrating motor complexes (CMMCs) in the proximal and distal colon and summary data of the area under the curve (AUC) at 300 s. The data are normalized to the control value (before the application of L-NAME) (n = 7; ^aP < 0.05 vs control; ^cP < 0.05 vs proximal colon). B and D: Enhanced effects of L-NAME on spontaneous contraction in the proximal and distal colon of mice and summary of the contractile responses to L-NAME as indicated by the AUC at 200 s. The data are normalized to the control value (before the application of L-NAME) (n = 7; ^aP < 0.05 vs control; ^cP < 0.05 vs proximal colon). E and G: Responses of CMMCs to NPPB in the proximal and distal colon and the summary of data as indicated by the AUC at 300 s. The data are normalized to the control value (before the application of NPPB) (n = 5; ^aP < 0.05 vs control; ^cP < 0.05 vs proximal colon). F and H: Inhibitory effects of NPPB (3 μmol/L) on spontaneous contraction in the proximal and distal colon and summary of the data showing contractile responses to NPPB as indicated by the AUC at 200 s. The data are normalized to the control value (before the application of NPPB) (n = 5; ^aP < 0.05 vs control; ^cP < 0.05 vs proximal colon). AUC: Area under the curve.