Zinc finger E-box-binding homeobox 1 mediates aerobic glycolysis via suppression of sirtuin 3 in pancreatic cancer

Figure 7 Zinc finger E-box binding homeobox-1 interacts with methyl-CpG binding domain protein 1 to suppress sirtuin 3 expression in pancreatic cancer cells. A: Methyl-CpG binding domain protein 1 (MBD1) and zinc finger E-box binding homeobox-1 (ZEB1) repressed sirtuin 3 (SIRT3) promoter luciferase activity, and when cotransfected into HEK-293T cells, SIRT3 promoter activity more dramatically decreased than transfecting MBD1 or ZEB1 alone; B and C: Chromatin immunoprecipitation (ChIP) assay demonstrated that MBD1 and ZEB1 bound the Z-box region of the SIRT3 promoter; D: ChIP and re-ChIP assays demonstrated that MBD1 and ZEB1 jointly occupied the same Z-box region in the SIRT3 promoter; E: Schematic representation of the working model. ^aP < 0.05, and ^bP < 0.01 vs NC or mock group. Error bars indicate mean ± SD. ChIP: Chromatin immunoprecipitation; MBD1: Methyl-CpG binding domain protein 1; SIRT3: Sirtuin 3; ZEB1: Zinc finger E-box binding homeobox-1.