Establishment, functional and genetic characterization of three novel patient-derived rectal cancer cell lines

Figure 2 Morphology and phenotype of individual rectal cancer cell lines. A: Light microscopy of freshly established tumor cell lines (all passage 6-11). Cell lines were established from patients’ tumor material as described in material and methods. Original magnification × 100; B: Phenotyping was conducted by flow cytometry (BD FACSARIA II) using fluorochrome-labeled mAbs as given on the x-axis. Exemplary data of one analysis out of three biological replicates are given. Some markers display a high variation in dependence from cell density in the culture vessels. HLA: Human leukocyte antigen.