Typing of pancreatic cancer-associated fibroblasts identifies different subpopulations

Figure 3 CD10 expression in pancreatic cancer. A: Semi-quantitative mean labelling scores of CD10 in juxtatumoural cancer-associated fibroblasts (j-CAFs) = 2.6, peripheral cancer-associated fibroblasts (p-CAFs) = 0.8, lobular fibroblasts = 1.4, and septal fibroblasts (s-FBs) = 0.9. CD10 is expressed at significantly higher levels in j-CAFs than in p-CAFs and s-FBs. ^bP < 0.01; B: Moderate CD10 expression in j-CAFs (scale bar, 100 μm); C: In p-CAFs, CD10 expression is only barely perceptible (scale bar, 250 μm). Maspin-positive cancer cells are; D: present in juxtatumoural stroma (scale bar, 100 μm) but; E: not in peripheral stroma (scale bar, 250 μm). Strong α-smooth muscle actin (α-SMA) expression in F: j-CAFs (scale bar, 100 μm); and G: p-CAFs (scale bar, 250 μm); H: Co-expression of CD10 and α-SMA in j-CAFs is shown [double-immunofluorescence (d-IF) of α-SMA (green) and CD10 (red); scale bar, 10 μm]. No co-expression of CD10 with; I: the endothelial marker ETS-related gene (ERG) [d-IF of ERG (green) and CD10 (red); scale bar, 10 μm]; or J: with the histiocyte marker IBA1 [d-IF of IBA1 (green) and CD10 (red); scale bar, 20 μm]. j-CAFs: Juxtatumoural cancer-associated fibroblasts; p-CAFs: Peripheral cancer-associated fibroblasts; l-FBs: Lobular fibroblasts; s-FBs: Septal fibroblasts.