Helicobacter pylori promotes invasion and metastasis of gastric cancer by enhancing heparanase expression

Figure 2 Heparanase protein expression following Helicobacter pylori infection in MKN-45 gastric cancer cells via the mitogen-activated protein kinase signaling pathway. A: Heparanase (HPA) expression was determined by Western blot at 0, 6, 12, 24, and 48 h after Helicobacter pylori (H. pylori) infection; B: Quantitative Western blot results of HPA; C: p-p38MAPK expression was determined by Western blot at 0, 30, 60, 120, and 480 min after H. pylori infection; D: Quantitative Western blot results of p-p38MAPK; E: HPA expression when the MAPK inhibitor SB203580 was given to MKN-45 cells before H. pylori infection; F: Quantitative Western blot results of HPA when the MAPK inhibitor SB203580 was given. ^bP < 0.01 compared with the value at 0 h. G, H: Cell invasion rates in the three groups detected using a Transwell invasion assay. I, J: Migration rates in the three groups detected using a scratch migration assay. ^aP < 0.05, ^bP < 0.01. HPA: Heparanase; MAPK: Mitogen-activated protein kinase; H. pylori: Helicobacter pylori.