Temporal clinical, proteomic, histological and cellular immune responses of dextran sulfate sodium-induced acute colitis

Figure 3 Colon and spleen in 3% dextran sulfate sodium time course. Experimental colitis was induced by the administration of DSS 3% in drinking water for 7 d. Colon shortening started as early as day 3 (A), with no changes in colon weight (B). Spleen weight was increased on day 6 and day 8 (C), at the same time as histological scores were augmented, demonstrating crypt depletion, loss of goblet cells, loss of the epithelial layer and inflammatory cells’ infiltration (D). One-way ANOVA followed by Dunnett post-hoc test. ^aP < 0.05 compared to control (day 0). n = 4/each time point for histology and n = 6/each time point for colon and spleen measurements. DSS: Dextran sulfate sodium.