Copyright
©The Author(s) 2018.
World J Gastroenterol. Oct 14, 2018; 24(38): 4356-4368
Published online Oct 14, 2018. doi: 10.3748/wjg.v24.i38.4356
Published online Oct 14, 2018. doi: 10.3748/wjg.v24.i38.4356
Table 1 Nucleotide sequences of forward and reverse primers
Gene | Forward primer (5´-3´) | Reverse primer (5´-3´) | Product length (bp) |
eNOS | 5´-AAGTGGGCAGCATCACCTAC-3´ | 5´-GCCTGGGAACCACTCCTTTT-3´ | 211 |
iNOS | 5´-CTCACTGGGACTGCACAGAA-3´ | 5´-TGTTGAAGGGTGTCGTGAAA-3´ | 128 |
PDE-5 | 5´-GCGGAGGAAGAAACAAGGGA-3´ | 5´-ATCGGCAAAGAACCTCGTGT-3´ | 196 |
sGCa1 | 5´-GCCCCACGACATACAGGTTA-3´ | 5´-GCGGCTCACTAATCTACCCC-3´ | 229 |
sGCb1 | 5´-AATTACGGTCCCGAGGTGTG-3´ | 5´-ACCAGCATTGAGGTTGAGGAC-3´ | 147 |
18sRNA (reference) | 5´-GTAACCCGTTGAACCCCATT-3´ | 5´-CCATCCAATCGGTAGTAGCG-3´ | 151 |
srsf4 (reference) | 5´-GGTTCTGGACGCAGTGGATA-3´ | 5´-CTCCTTCGTTTTTGCGTCCC-3´ | 193 |
- Citation: Schaffner D, Lazaro A, Deibert P, Hasselblatt P, Stoll P, Fauth L, Baumstark MW, Merfort I, Schmitt-Graeff A, Kreisel W. Analysis of the nitric oxide-cyclic guanosine monophosphate pathway in experimental liver cirrhosis suggests phosphodiesterase-5 as potential target to treat portal hypertension. World J Gastroenterol 2018; 24(38): 4356-4368
- URL: https://www.wjgnet.com/1007-9327/full/v24/i38/4356.htm
- DOI: https://dx.doi.org/10.3748/wjg.v24.i38.4356