Mechanism of combined use of vitamin D and puerarin in anti-hepatic fibrosis by regulating the Wnt/β-catenin signalling pathway

Figure 3 The protein levels of Wnt1 and β-catenin of liver tissues in rats in the CCl₄ group and the medicated groups were higher than the Control (C) group. A: Detection of protein levels of Wnt1, and β-catenin using western blot assay; B: Grey analysis of Wnt1; C: Grey analysis of β-catenin [in the comparison of mean ± standard deviation (SD) of various groups, differences are statistically significant when P < 0.05, n = 10. In addition, a, b, c, d, and e represent statistical differences vs C, CCl₄, V, P, and V + P groups, respectively]. C: Control; V: Vitamin; P: Puerarin.