Formin-like 3 regulates RhoC/FAK pathway and actin assembly to promote cell invasion in colorectal carcinoma

Figure 1 Forced expression of FMNL3 promotes colorectal carcinoma cell proliferation, invasion, and migration in vitro. A and B: Identification of FMNL3 expression in FMNL3-overexpressing cells by real-time quantitative PCR and western blot. C: Effects of FMNL3 overexpression on cell proliferation by MTT assay. D: Effects of FMNL3 overexpression on invasive abilities by Boyden chamber assay. Morphological comparison of cell penetration into the artificial basement membrane is shown. E: Effects of FMNL3 overexpression on migratory abilities by scratch assay in vitro. Scale bars represent 50 μm (cell invasion assay) or 100 μm (cell migration assay), respectively. ^aP < 0.001, ^bP < 0.01 and ^cP < 0.05 vs NC or mock group. Error bars indicate mean ± SD.