Optimal immunosuppressor induces stable gut microbiota after liver transplantation

Figure 6 Phylogenetic tree analysis of sequences in the denaturing gradient gel electrophoresis profiles. Phylogenetic tree analysis of sequences from denaturing gradient gel electrophoresis (DGGE) profiles using the neighbor-joining method was conducted using MEGA 5 software. Almost all matched bacteria of the 48 DGGE band classes were assigned to three phyla: Bacteroidetes (50.0%), Firmicutes (39.6%), and Gammaproteobacteria (10.4%). The fragment sequences were defined according to their positions in gels using the band-matching tool with BioNumerics software version 6.01 (Applied Maths). The numbers in the brackets were consistent with the numbers shown in DGGE profiling. Nine band classes (labeled with a red dot) were decreased and four band classes (labeled with the green square) were higher in the FK506-M group than the rejection group. The plot was obtained using MEGA5 software (http://en.wikipedia.org/wiki/MEGA,_Molecular_Evolutionary_Genetics_Analysis).