Delta-like ligand 4 in hepatocellular carcinoma intrinsically promotes tumour growth and suppresses hepatitis B virus replication

Figure 3 Suppression of Delta-like ligand 4 enhances hepatitis B virus viral replication (A), HBx mRNA expression (B), and HBs protein expression (C) in vivo. DNA, RNA, and proteins were extracted from tumour xenografts to analyse HBV viral components at 18 d and 30 d after implantation. The mRNA levels of human IFN-alpha (D), IFN-beta (E), and TNF-alpha (F) from tumours transfected with shDLL4 or control vectors were measured by quantitative RT-PCR and normalized to beta-actin mRNA expression. The data are represented by the mean ± SD. ^aP < 0.05; ^bP < 0.01.