Impact of hyperglycemia on autoimmune pancreatitis and regulatory T-cells

Figure 5 Evaluation of leukocytes in the spleen during autoimmune pancreatitis. Representative images of stained T-helper cells and their distinction between regulatory T-cells (FoxP3⁺CD4⁺ cells), CD25 expressing regulatory T-cells (CD4⁺CD25⁺ FoxP3⁺ cells) and FoxP3- T-cells (FoxP3^- CD4⁺ cells) (A); application of STZ decreased the percentage of T-lymphocytes (defined as number of CD3⁺ cells × 100 divided by the number of lymphocytes) (B); little differences are observed in the percentage of cytotoxic T-cells (CD8⁺CD3⁺ cells × 100 divided by the number of CD3⁺cells) (C); and T-helper cells (CD4⁺CD3⁺ cells × 100 divided by the number of CD3⁺cells) (D); STZ increased the percentage of regulatory T-cells (FoxP3⁺CD4⁺cells × 100 divided by the number of CD4⁺ cells) (E); and CD25 expressing regulatory T-cells (FoxP3⁺ CD25⁺ CD4⁺ cells × 100 divided by the number of CD4⁺cells) (F); the percentage of FoxP3^- T-cells (FoxP3^-CD4⁺cells × 100 divided by the number of CD4⁺ cells) was decreased (G). The number of animals evaluated was n = 19 (sham) and n = 17 (STZ). Differences between the indicated cohorts are indicated as tendency, (B) ^aP = 0.057 or as significant, (E) ^bP = 0.018; (F) ^cP = 0.021; (G) ^dP = 0.034. STZ: Streptozotocin-treated.