Association of twelve polymorphisms in three onco-lncRNA genes with hepatocellular cancer risk and prognosis: A case-control study

Table 5 Differences of lncRNA gene mRNA levels in different genotypes in hepatocellular cancer and non-cancer tissues

Variable	Non-cancer tissues				Cancer tissues
	n	ΔCt (Mean ± SD)	Normalized 2-ΔΔCt	P1 value	n	ΔCt (Mean ± SD)	Normalized 2-ΔΔCt	P1 value
The effect of HOTTIP rs17501292 genotypes to HOTTIP mRNA expression
TT	10	12.32 ± 4.06	1 (0.60, 16.68)	Ref.	25	11.84 ± 3.87	1.39 (0.10, 20.39)	Ref.
TG	1	NA	NA	NA	2	15.30 ± 1.65	NA	0.735
GG	0	NA	NA	NA	0	NA	NA	NA
The effect of HOTTIP rs2067087 genotypes to HOTTIP mRNA expression
GG	2	8.64 ± 2.06	1 (0.24, 4.17)	Ref.	5	11.66 ± 1.43	1 (0.37, 2.69)	Ref.
GC	5	12.25 ± 4.17	0.13 (0.01, 1.23)	0.253	13	11.85 ± 3.53	0.88 (0.08, 10.13)	0.101
CC	4	13.70 ± 3.86	0.03 (0.00, 0.86)	0.453	8	12.23 ± 5.42	0.67 (0.02, 28.84)	0.444
The effect of HOTTIP rs17427960 genotypes to HOTTIP mRNA expression
CC	2	8.64 ± 2.06	1 (0.24, 4.17)	Ref.	6	12.47 ± 2.34	1 (0.20, 5.06)	Ref.
CA	5	11.55 ± 3.21	0.13 (0.01, 1.15)	0.254	13	11.88 ± 3.72	2.13 (0.04, 125.37)	0.063
AA	3	13.56 ± 4.71	0.03 (0.00, 0.86)	0.465	6	11.38 ± 5.88	1.51 (0.11, 19.84)	0.348
The effect of MALAT1 rs4102217 genotypes to MALAT1 mRNA expression
GG	52	-2.25 ± 4.40	1 (0.05, 21.11)	Ref.	51	-0.98 ± 4.88	1 (0.03, 29.45)	Ref.
GC	16	-3.76 ± 3.60	2.85 (0.23, 34.54)	0.404	16	-2.79 ± 4.55	3.51 (0.15, 82.14)	0.742
CC	NA	NA	NA	NA	1	NA	NA	NA

¹The statistical analysis for the effect of genotype to phenotype was used two-independent sample t-test, and for the combination of genotype to phenotype was used ANOVA analysis. NA: Not available.