β-arrestin 2 attenuates lipopolysaccharide-induced liver injury via inhibition of TLR4/NF-κB signaling pathway-mediated inflammation in mice

Figure 3 Expression of inflammatory factors by RAW264. 7 cells in vitro. A: Expression of β-arrestin 2 in RAW264.7 cells was detected by Western blot. Levels of GAPDH are shown as a loading control; B: Relative quantitative evaluation of the Western blot analysis for β-arrestin 2 expression with ImageJ software, C-F: qRT-PCR was used to determine relative mRNA levels of IL-1β (C), IL-6 (D), TNF-α (E), and IL-10 (F) produced by RAW264.7 cells. ^aP < 0.05 vs vehicle group; ^cP < 0.05 vs control siRNA group.