Antifibrogenic effects of vitamin D derivatives on mouse pancreatic stellate cells

Figure 5 Effects of D-vitamins on pancreatic stellate cell gene expression (A-D). Pancreatic stellate cells of the first passage were treated for 48 h with the indicated D-vitamins at 100 nmol/L each. Subsequently, the mRNA expression of the indicated genes and the house-keeping control Hprt was analyzed by real-time PCR, and relative amounts of target mRNA were calculated as described in the methods section. One hundred percent mRNA expression of each gene corresponds to untreated cells. Data are presented as averaged mean ± SE (n ≥ 8 independent samples). ^aP < 0.05 vs untreated controls.