Antifibrogenic effects of vitamin D derivatives on mouse pancreatic stellate cells

Figure 3 D-vitamins reduce protein levels of α-smooth muscle actin in primary cultured pancreatic stellate cells. Pancreatic stellate cells were treated with the indicated D-vitamins (100 nmol/L each) from day 1-4 of primary culture, before expression of α-smooth muscle actin (α-SMA) and the housekeeping protein GAPDH was assessed by Western blot analysis (see A for a typical experiment); B: Intensities of fluorescence signals were expressed as ratio α-SMA/GAPDH. Data of 5 independent experiments were used to calculate mean ± SE. ^aP < 0.05 vs untreated controls.