Antifibrogenic effects of vitamin D derivatives on mouse pancreatic stellate cells

Figure 1 D-vitamins prevent loss of lipid droplets in quiescent pancreatic stellate cells. Pancreatic stellate cells in primary culture (days 1-4) and of passage 1 (days 1-3) were exposed to the indicated D-vitamins at 100 nmol/L each. Afterwards, intracellular lipids were stained with oil-red, and size/number of lipid droplets were assessed on a semiquantitative scale from 0 to 3. A: Exemplary stains for scores 1-3 (original magnification, × 400); B: Shown are the mean scores of n ≥ 14 independent samples (mean ± SE) for primary cultures (left panel) and passaged cells (right panel). ^aP < 0.05 vs untreated controls.