Ischemia/reperfusion injury in porcine intestine - Viability assessment

Figure 5 Transmission electron microscopy of jejunum (muscularis propria) sampled at selected time intervals of ischemia and reperfusion. Images are indexed with I = ischemia hours and R = reperfusion hours. 0: Intact muscle. I-1: Mild intercellular edema, with increased variation in the electron density in the muscle cells. Some minimal fat vacuoles are visible. I-1 R-3: Focal/single cell necrosis with inflammatory response, low grade fine-vacuolization of the sarcoplasm. I-3 R-3: Active interstitial inflammation, swollen muscle cell nuclei. I-4 R-3: Severe interstitial edema and loss of coherence among muscle cells. Swollen nuclei and focal, mostly single cell necrosis. I-5 R-3: Focal multi cell necrosis, interstitial inflammation, vacuolization of sarcoplasm. I-4 R-3*: Swollen nucleus (A), vacuolated sarcoplasm (B) and swollen mitochondria (C). I-5 R-3*: Necrotic muscle cell adjacent to a more intact cell with some vacuoles (B) and slightly swollen mitochondria (C).