Mitochondrial pathway mediated by reactive oxygen species involvement in α-hederin-induced apoptosis in hepatocellular carcinoma cells

Figure 4 α-Hederin inhibits tumor growth in vivo. Mice with xenograft tumors were divided into four groups (Control and 2.5 mg/kg, 5 mg/kg and 10 mg/kg α-Hederin, n = 6 mice per group). A: Mean tumor volume at each time point and final tumor weight, P for trend < 0.05; B: TUNEL assays detected apoptotic cells in xenograft tumor tissue, as evidenced by the presence of nut-brown nuclei under a fluorescence microscope. The percentage of apoptotic cells was calculated, P for trend < 0.05. ^aP < 0.05 vs control.