Oral treatment with plecanatide or dolcanatide attenuates visceral hypersensitivity via activation of guanylate cyclase-C in rat models

Figure 3 Effect of plecanatide and dolcanatide on localization of occludin in epithelial cells. Caco-2 (A) and T84 (B) cell monolayers were treated with 1 μmol/L plecanatide or dolcanatide in the presence or absence of 100 μg/mL of LPS for 16 h followed by immunofluorescence imaging for occludin. Representative microscopic fields demonstrate disruption of occludin localization by LPS. Co-treatment of LPS with plecanatide or dolcanatide preserved occludin localization around the cell membrane, as was observed for vehicle treated cells. Images taken at 40 × resolution. Blue fluorescence corresponds to DAPI stained nucleus. DAPI: 4’, 6’-diamidino-2-phenylindole; LPS: lipopolysaccharide.