Oral treatment with plecanatide or dolcanatide attenuates visceral hypersensitivity via activation of guanylate cyclase-C in rat models

Figure 1 Effect of plecanatide and dolcanatide on lipopolysaccharide-induced increase in permeability of 4 kDa fluorescein isothiocyanate-dextran across Caco-2 and T84 cell monolayers. Caco-2 (A and B) and T84 (C and D) cells cultured on snap well inserts were treated with vehicle or 1 μmol/L of plecanatide (A and C) or dolcanatide (B and D) in the presence or absence of 100 μg/mL of LPS for 16 h. Subsequently, 1 mg/mL of FITC-dextran was added to the apical compartment. Paracellular permeability was determined by measuring the amount of FITC-dextran present in the basal compartment. Data represent mean ± SEM analyzed in triplicates. D: Dolcanatide; FITC: Fluorescein isothiocyanate; LPS: Lipopolysaccharide; P: Plecanatide; RFU: Relative fluorescence units; SEM: Standard error of the mean; V: Vehicle.