Emergence of immunotherapy as a novel way to treat hepatocellular carcinoma

Figure 6 Preparation of genetically modified TCR-expressing T cells. TCRα and TCRβ genes are cloned and amplified with the use of PCR from TAA-specific T cells, which are isolated and expanded ex vivo. The obtained TCRα and TCRβ genes are cloned into an expression vector, which is used for transfection into T cells. The transfected cells are adoptively transferred after ex vivo expansion.TAA: Tumor-associated antigen.