Mucosa repair mechanisms of Tong-Xie-Yao-Fang mediated by CRH-R2 in murine, dextran sulfate sodium-induced colitis

Figure 2 Inhibition of CRH-R2 signaling increases secretion of inflammatory cytokines in colon tissues of DSS-induced ulcerative colitis mice. A-C: Real time-PCR-assessed mRNA level of TNF α (A), CXCL-1 (B) and IL 6 (C) in colon tissues. The mRNA level in each group was determined relative to the level in the control group (defined as 100%); E-F: Enzyme-linked immunosorbent assay-detected protein levels of TNF α (D), CXCL-1 (E) and IL 6 (F) in colon tissues. Data are presented as mean ± standard deviation, n = 6 per group. ^bP < 0.01, ^cP < 0.001 vs control group; ^dP < 0.05, ^eP < 0.01, ^fP < 0.001 vs DSS group; ^qP < 0.01, ^rP < 0.001 vs Ucn2 group; ^mP < 0.05, ⁿP < 0.01, ^oP < 0.001 vs TXYF-L group; ^gP < 0.05, ^hP < 0.01, ⁱP < 0.001 vs TXYF-M group; ^jP < 0.05, ^kP < 0.01, ^lP < 0.001 vs TXYF-H group. CRH-R2: Corticotropin-releasing hormone-receptor 2; DSS: Dextran sulfate sodium; TXYF: Tong-Xie-Yao-Fang.