Nonalcoholic steatohepatitis severity is defined by a failure in compensatory antioxidant capacity in the setting of mitochondrial dysfunction

Figure 10 Hepatic oxidative stress is mitigated by increased catalase activity in FATZO but not ob/ob mice. (A): Expression of antioxidant genes Nrf2, Sod1, Sod2, Cat and Gpx1 in ob/ob LFD, FATZO LFD, ob/ob AMLN and FATZO AMLN livers relative to lean controls. Superoxide dismutase activity (B) and catalase activity (C) in hepatic lysates from C57BL6J, ob/ob LFD, FATZO LFD, ob/ob AMLN and FATZO AMLN animals; (D): Representative images of catalase stained liver sections. Scale bar represents 100 μm; (E): Ratio of catalase: SOD activities; (F): Levels of 8-hydroxydeoxyguanosine (8-OHdG) in genomic DNA isolated from C57BL6J, ob/ob LFD, FATZO LFD, ob/ob AMLN and FATZO AMLN livers; (G): Pearson’s correlation of catalase activity and 8-OHdG levels in ob/ob and FATZO livers. ^aP ≤ 0.05, ^bP ≤ 0.01, ^cP ≤ 0.001, ^dP ≤ 0.0001 vs C57BL6J; ^jP ≤ 0.01, ^kP ≤ 0.001, FATZO LFD; ^mP ≤ 0.05, ⁿP ≤ 0.01, FATZO AMLN unless noted otherwise. LFD: Low-fat diet.