Copyright
©The Author(s) 2018.
World J Gastroenterol. Mar 28, 2018; 24(12): 1299-1311
Published online Mar 28, 2018. doi: 10.3748/wjg.v24.i12.1299
Published online Mar 28, 2018. doi: 10.3748/wjg.v24.i12.1299
Table 3 Primers and templates for overlap PCR
| Fragment | Template | Primer | |
| Upstream | Downstream | ||
| mE2 | mE2-1 + mE2-2 | 1340-F | 3500-R |
| mp7 | mp7-1 + mp7-2 | 1340-F | 3500-R |
| mNS4B | mNS4B-1 + mNS4B-2 | 5249 F | 7791-R |
| mNS5A-3/4 | mNS5A-3 + mNS5A-4 | m7359-F | 7966-R |
| mNS5A-2/3/4 | mNS5A-2 + mNS5A-3/4 | m7160-F | 7966-R |
| mNS5A | mNS5A-1 + mNS5A-2/3/4 | 5249-F | 7966-R |
| mNS4B/NS5A | mNS4B/NS5A-1 + mNS4B/NS5A-2 | 5249 F | 7791-R |
| mE2/p7 | mE2/p7 -1 + mE2/p7-2 | 1340-F | 3500-R |
- Citation: Wang Q, Li Y, Liu SA, Xie W, Cheng J. Cell culture-adaptive mutations in hepatitis C virus promote viral production by enhancing viral replication and release. World J Gastroenterol 2018; 24(12): 1299-1311
- URL: https://www.wjgnet.com/1007-9327/full/v24/i12/1299.htm
- DOI: https://dx.doi.org/10.3748/wjg.v24.i12.1299