Copyright
©The Author(s) 2018.
World J Gastroenterol. Mar 28, 2018; 24(12): 1299-1311
Published online Mar 28, 2018. doi: 10.3748/wjg.v24.i12.1299
Published online Mar 28, 2018. doi: 10.3748/wjg.v24.i12.1299
Table 2 Primer combinations used in adaptive mutation plasmid construction
| Fragment | Template | Primers | |
| Sense | Anti-sense | ||
| mE2-1 | JFH1 | 1340-F | m2310-R |
| mE2-2 | m2310-F | 3500-R | |
| mp7-1 | 1340-F | m2681-R | |
| mp7-2 | m2681-F | 3500-R | |
| mNS4B-1 | 5249-F | m6132-R | |
| mNS4B-2 | m6132-F | 7791-R | |
| mNS5A-1 | 5249-F | m7160-R | |
| mNS5A-2 | m7160-F | m7359-R | |
| mNS5A-3 | m7359-F | m7658-R | |
| mNS5A-4 | m7658-F | 7966-R | |
| mNS4B/NS5A-1 | JFH1-mNS5A | 5249-F | m6132-R |
| mNS4B/NS5A-2 | m6132-F | 7791-R | |
| mE2/p7 -1 | JFH1-mp7/NS5A | 1350-F | m2310-R |
| mE2/p7 -2 | m2310-F | 3500-R | |
- Citation: Wang Q, Li Y, Liu SA, Xie W, Cheng J. Cell culture-adaptive mutations in hepatitis C virus promote viral production by enhancing viral replication and release. World J Gastroenterol 2018; 24(12): 1299-1311
- URL: https://www.wjgnet.com/1007-9327/full/v24/i12/1299.htm
- DOI: https://dx.doi.org/10.3748/wjg.v24.i12.1299