Increased intestinal mucosal leptin levels in patients with diarrhea-predominant irritable bowel syndrome

Figure 4 Double-labeling immunofluorescence of leptin receptor with tryptase and PGP9. 5 in irritable bowel syndrome patients and healthy controls (×400 magnification). A: Colonic mucosal double-labeling immunofluorescence of leptin receptor and tryptase in IBS-D patients (× 400, arrows represent colocalization of leptin receptor and tryptase); B: Colonic mucosal double-labeling immunofluorescence of leptin receptor and tryptase in healthy controls (× 400, arrows represent colocalization of leptin receptor and tryptase); C: Colonic mucosal double-labeling immunofluorescence of leptin receptor and PGP9.5 in IBS-D patients (× 400, arrows represent colocalization of leptin receptor and PGP9.5); D: Colonic mucosal double-labeling immunofluorescence of leptin receptor and PGP9.5 in healthy controls (× 400, arrows represent colocalization of leptin receptor and PGP9.5). PGP9.5: Protein gene product 9.5; IBS-D: Iirritable bowel syndrome with diarrhea.