Copyright
©The Author(s) 2017.
World J Gastroenterol. Mar 7, 2017; 23(9): 1568-1575
Published online Mar 7, 2017. doi: 10.3748/wjg.v23.i9.1568
Published online Mar 7, 2017. doi: 10.3748/wjg.v23.i9.1568
Table 2 List of primers used in this study
| Pyrosequencing | Forward | Reverse | Ta (°C) | MgCl2 (mmol/L) | Sequencing |
| miR-23 cluster | TTTAAGTYGTGTGAAATTATGTGGTAG | ATAAACACCRAAAAACRAATCCA | 60 | 2.5 | TGGTAGTTTATGGTTGTGAG |
| miR-25 cluster | GTGYGGGTTAATYGGATAAGG | AAAAACCCRACRCCTACACTAC | 60 | 2.5 | GYGGGTTTAGAATGAGT |
| miR-183 | GTTATTAATAGGAATGGGGTAG | AAACRACTCTCAACCTCCC | 60 | 1.5 | GAATGGGGTAGTTGAGGG |
- Citation: Anwar SL, Krech T, Hasemeier B, Schipper E, Schweitzer N, Vogel A, Kreipe H, Buurman R, Skawran B, Lehmann U. hsa-mir-183 is frequently methylated and related to poor survival in human hepatocellular carcinoma. World J Gastroenterol 2017; 23(9): 1568-1575
- URL: https://www.wjgnet.com/1007-9327/full/v23/i9/1568.htm
- DOI: https://dx.doi.org/10.3748/wjg.v23.i9.1568