Different phenotypes of monocytes in patients with new-onset mild acute pancreatitis

Figure 3 Flow cytometry analysis of peripheral blood CD206+ and CD115+ M2 monocytes. Peripheral blood mononuclear cells (PBMCs) were isolated from individual subjects and stained in duplicate with BV510-anti-CD14, PE/Cy7-anti-CD163, PE-anti-CD115, and APC/Cy7-anti-206 or isotype controls. The numbers of peripheral blood CD14+CD163+CD206+, CD14+CD163+CD115+ and CD14+CD163+CD206+CD115+ M2 monocytes were analyzed by flow cytometry. Data shown are representative FACS charts and expressed as the mean numbers of each type of cells per mL of blood in individual subjects. The horizontal lines indicate the median values for each group. FSC: Forward scatter; SSC: Side scatter; HC: Healthy control; MAP: Mild acute pancreatitis.