Different phenotypes of monocytes in patients with new-onset mild acute pancreatitis

Figure 2 Flow cytometry analysis of peripheral blood MAC387+ monocytes. Peripheral blood mononuclear cells (PBMCs) were isolated from individual subjects and stained in duplicate with BV510-anti-CD14 and PE/Cy7-anti-CD163 or isotype controls. After being washed, the cells were fixed and permeabilized using a fixation/permeabilization kit, followed by intracellular staining with FITC-anti-MAC387. The numbers of peripheral blood CD14+CD163+MAC387+ and CD14+CD163-MAC387+ monocytes were analyzed by flow cytometry. Data are representative FACS charts and expressed as the mean numbers of each type of cells in individual subjects. The horizontal lines indicate the median values for each group. FSC: Forward scatter; SSC: Side scatter; HC: Healthy control; MAP: Mild acute pancreatitis.