Caffeic acid phenethyl ester up-regulates antioxidant levels in hepatic stellate cell line T6 via an Nrf2-mediated mitogen activated protein kinases pathway

Figure 6 Effect of inhibitors of mitogen activated protein kinases and caffeic acid phenethyl ester on the antioxidant-related indicator protein and mRNA expression. A: Effect of inhibitors of MAPKs and CAPE on the SOD activity and GSH and CAT content in HSC-T6 cells. Cells were treated with the ERK1/2 inhibitor PD98059 (30 μmol/L), p38MAPK inhibitor SB203580 (20 μmol/L) or JNK inhibitor SP600125 (25 μmol/L) for 2 h, then incubated with CAPE (15 μmol/L) for 24 h; B: Effect of inhibitors of MAPKs and CAPE on antioxidant-related mRNA expression in HSC-T6 cells. The data represent averages of the results of three independent experiments. ^aP < 0.05 vs control; ^cP < 0.05 vs CAPE group. CAPE: Caffeic acid phenethyl ester; SOD: Superoxide dismutase; CAT: Catalase; GST: Glutathione-S-transferase.