Thymoquinone suppresses migration of LoVo human colon cancer cells by reducing prostaglandin E2 induced COX-2 activation

Figure 2 Thymoquinone inhibits the expression of PI3K, Akt, p-GSK3β, β-catenin and COX-2 proteins in LoVo cells. LoVo cells were cultured in medium that was treated with TQ (5, 10 and 20 μmol/L) for 24 h; PGE2 (5 μmol/L) was added for the last 6 h. Following this, the cells were immunoblotted with the indicated antibodies. The cells were lysed, and the extracts were separated by 10% SDS-PAGE, transferred to PVDF membranes and immunoblotted with antibodies against p-PI3K, p-Akt, p-GSK3β, β-catenin and COX-2.