Copyright
©The Author(s) 2017.
World J Gastroenterol. Feb 21, 2017; 23(7): 1203-1214
Published online Feb 21, 2017. doi: 10.3748/wjg.v23.i7.1203
Published online Feb 21, 2017. doi: 10.3748/wjg.v23.i7.1203
Antibody (dilution) | Clone | Species | Specificity | Source |
Nrf2 | M | Rabbit | Immunogen: ag9489 | ProteinTech, United States |
(16396-1-AP 1:500) | ||||
P38MAPK | M | Rabbit | Synthetic peptide corresponding to residues near the C-terminus of human p38 MAP kinase was used as immunogen. Predicted to react with CSBP1 splice form based on sequence homology | EPITOMICS, United States |
(ab32142, 1:500) | ||||
P-p38MAPK | M | Rabbit | Phospho-P38 MAPKinase (Thr180/Tyr182) (3D7) rabbit mAb detects endogenous levels of p38MAP kinase only when dually phosphorylated at Thr180 and Tyr182. This antibody does not cross-react with the phosphorylated forms of either p42/44 MAPK or SAPK/JNK | Cell Signaling, United States |
(#4511, 1:500) | ||||
Erk1/2 | M | Rabbit | P44/42 MAP kinase (137F5) rabbit mAb detects endogenous level of total P44/42 MAP kinase (Erk1/Erk2) protein. The antibody does not cross-react with JNK/SAPK or p38 MAP kinase | Cell Signaling, United States |
(#4695, 1:500) | ||||
Erk1phospho(pY204)/Erk2phospho | M | Rabbit | A phospho-specific peptide corresponding to residues surrounding tyrosine 204 of human Erk1. This antibody detects Erk1 phosphorylated at tyrosine 204 | EPITOMICS, United States |
(pY187) | ||||
(ab76299 1:500) | ||||
SAPK/JNK | M | Rabbit | SAPK/JNK(56G8) rabbit mAb detects endogenous levels of total SAPK/JNK protein | Cell Signaling, United States |
(#9258 1:500) | ||||
JNK1phospho(pT183) /JNK2phospho(pT221) (ab124956 1:500) | M | Rabbit | A phospho-specific peptide corresponding to residues surrounding threonine 221 of human JNK3 was used as immunogen. The antibody detects JNK1 (pT183), JNK2 (pT183) and JNK3 (pT221) | EPITOMICS, United States |
α-SMA | M | Rabbit | A synthetic peptide corresponding to N-terminus of human actin was used as immunogen | EPITOMICS, United States |
(ab124964,1: 1000) | ||||
Collegen-1 | P | Rabbit | KLH conjugated synthetic peptide derived from human Collagen I C-terminal propeptide | Bioss, China |
(bs-0578R 1:500) | ||||
β-actin | M | Mouse | β-actin is recommended for detection of β-actin of mouse, rat, human, chicken, dog, pig, rabbit | SANTA CRUZ, United States |
(sc-47778, | ||||
1:1000) |
- Citation: Yang N, Shi JJ, Wu FP, Li M, Zhang X, Li YP, Zhai S, Jia XL, Dang SS. Caffeic acid phenethyl ester up-regulates antioxidant levels in hepatic stellate cell line T6 via an Nrf2-mediated mitogen activated protein kinases pathway. World J Gastroenterol 2017; 23(7): 1203-1214
- URL: https://www.wjgnet.com/1007-9327/full/v23/i7/1203.htm
- DOI: https://dx.doi.org/10.3748/wjg.v23.i7.1203