Basic Study
Copyright ©The Author(s) 2017.
World J Gastroenterol. Feb 21, 2017; 23(7): 1203-1214
Published online Feb 21, 2017. doi: 10.3748/wjg.v23.i7.1203
Table 2 Antibodies used for Western blot analysis
Antibody (dilution)CloneSpeciesSpecificitySource
Nrf2MRabbitImmunogen: ag9489ProteinTech, United States
(16396-1-AP 1:500)
P38MAPKMRabbitSynthetic peptide corresponding to residues near the C-terminus of human p38 MAP kinase was used as immunogen. Predicted to react with CSBP1 splice form based on sequence homologyEPITOMICS, United States
(ab32142, 1:500)
P-p38MAPKMRabbitPhospho-P38 MAPKinase (Thr180/Tyr182) (3D7) rabbit mAb detects endogenous levels of p38MAP kinase only when dually phosphorylated at Thr180 and Tyr182. This antibody does not cross-react with the phosphorylated forms of either p42/44 MAPK or SAPK/JNKCell Signaling, United States
(#4511, 1:500)
Erk1/2MRabbitP44/42 MAP kinase (137F5) rabbit mAb detects endogenous level of total P44/42 MAP kinase (Erk1/Erk2) protein. The antibody does not cross-react with JNK/SAPK or p38 MAP kinaseCell Signaling, United States
(#4695, 1:500)
Erk1phospho(pY204)/Erk2phosphoMRabbitA phospho-specific peptide corresponding to residues surrounding tyrosine 204 of human Erk1. This antibody detects Erk1 phosphorylated at tyrosine 204EPITOMICS, United States
(pY187)
(ab76299 1:500)
SAPK/JNKMRabbitSAPK/JNK(56G8) rabbit mAb detects endogenous levels of total SAPK/JNK proteinCell Signaling, United States
(#9258 1:500)
JNK1phospho(pT183) /JNK2phospho(pT221) (ab124956 1:500)MRabbitA phospho-specific peptide corresponding to residues surrounding threonine 221 of human JNK3 was used as immunogen. The antibody detects JNK1 (pT183), JNK2 (pT183) and JNK3 (pT221)EPITOMICS, United States
α-SMAMRabbitA synthetic peptide corresponding to N-terminus of human actin was used as immunogenEPITOMICS, United States
(ab124964,1: 1000)
Collegen-1PRabbitKLH conjugated synthetic peptide derived from human Collagen I C-terminal propeptideBioss, China
(bs-0578R 1:500)
β-actinMMouseβ-actin is recommended for detection of β-actin of mouse, rat, human, chicken, dog, pig, rabbitSANTA CRUZ, United States
(sc-47778,
1:1000)