Caffeic acid phenethyl ester up-regulates antioxidant levels in hepatic stellate cell line T6 via an Nrf2-mediated mitogen activated protein kinases pathway

Table 1 Primers used for real-time PCR analysis

Gene	Primer sequence	Tm (°C)	Size (bp)	Source
Nrf2
F	5’-CTGCCATTAGTCAGTCGCTCTC-3’	57.3	22	Rat
R	5’-TCGGCTGGGACTTGTGTTC-3’	57.7	19	Rat
SOD1
F	5’-GCTTCTGTCGTCTCCTTGCT-3’	55.7	20	Rat
R	5’-CTCGAAGTGAATGA CGCCCT-3’	55.8	20	Rat
CAT
F	5’-TGGCTATGGCTCACACACCTTC-3’	59.3	22	Rat
R	5’-GAGGCCA TAATCCGGGTCTTC-3’	57.7	21	Rat
GSTs
F	5’-GTGGAGATTGACGGGATGAA -3’	54.2	20	Rat
R	5’-CGGTCTTGGCTTCTCTTTGG -3’	56.0	20	Rat
β-actin
F	5’-GGAGATTACTGCCCTGGCTCCTA-3’	60.2	23	Rat
R	5’-GACTCATCGTACTCCTGCTTGCTG-3’	59.3	24	Rat