Copyright
©The Author(s) 2017.
World J Gastroenterol. Feb 21, 2017; 23(7): 1189-1202
Published online Feb 21, 2017. doi: 10.3748/wjg.v23.i7.1189
Published online Feb 21, 2017. doi: 10.3748/wjg.v23.i7.1189
ID | Primer | Sequence (5'to3') | Base (bp) | Tm (°C) | GC |
HIPK2 | Forward | CCCCGTGTACGAAGGTATGG | 20 | 59.90 | 60% |
Reverse | GGGATGTTCTTGCTCTGGCT | 20 | 60.03 | 55% | |
PDE3B | Forward | TGAGAGTTATGGCTGCCTGT | 20 | 58.72 | 50% |
Reverse | CTGAGGGGCATTTGTAGCCA | 20 | 60.30 | 55% | |
FGF2 | Forward | TCCACCTATAATTGGTCAAAGTGGT | 25 | 59.99 | 40% |
Reverse | CATCAGTTACCAGCTCCCCC | 20 | 59.82 | 60% | |
TWIST1 | Forward | ATTCAAAGAAACAGGGCGTGG | 21 | 59.39 | 47.6% |
Reverse | CAGAGGTGTGAGGATGGTGC | 20 | 60.39 | 40% | |
ZEB2 | Forward | GCCTCTGTAGATGGTCCAGTGA | 22 | 61.21 | 54.6% |
Reverse | ATCGCGTTCCTCCAGTTTTCT | 21 | 60.00 | 47.6% | |
VEGFC | Forward | CCCGCCTCTCCAAAAAGCTA | 20 | 60.04 | 55% |
Reverse | CGGGTGTCAGGTAAAAGCCT | 20 | 59.96 | 55% | |
SPHK1 | Forward | GCTGCGAAGTTGAGCGAAAA | 20 | 60.04 | 50% |
Reverse | CGTTCCCTACAGTGGCCTG | 19 | 60.08 | 63.2% | |
BAX | Forward | GCCCTTTTGCTTCAGGGTTT | 20 | 59.24 | 50% |
Reverse | CATCCTCTGCAGCTCCATGT | 20 | 59.82 | 55% | |
PTEN | Forward | CAGGATACGCGCTCGGC | 17 | 60.73 | 70.6% |
Reverse | ACAGCGGCTCAACTCTCAAA | 20 | 57.89 | 50% | |
HTRA1 | Forward | AGCCAAAGAGCTGAAGGACC | 20 | 59.96 | 55% |
Reverse | GACATCATTGGCGGAGACCA | 20 | 60.11 | 55% | |
CCL5 | Forward | TGCTGCTTTGCCTACATTGC | 20 | 59.76 | 50% |
Reverse | CTTGTTCAGCCGGGAGTCAT | 20 | 60.04 | 55% | |
TGM2 | Forward | CCTCTGTCTCTCCGGGAACC | 20 | 61.32 | 65% |
Reverse | TGGCAACCAGGGGTCCTAT | 19 | 60.23 | 57.9% | |
TLR4 | Forward | CTCGGTCAGACGGTGATAGC | 20 | 59.97 | 60% |
Reverse | TTTAGGGCCAAGTCTCCACG | 20 | 59.68 | 55% | |
ACTB | Forward | CTCACCATGGATGATGATATCGC | 23 | 59.13 | 47.8% |
Reverse | AGGAATCCTTCTGACCCATGC | 21 | 59.79 | 52.4% |
- Citation: Xie XQ, Zhao QH, Wang H, Gu KS. Dysregulation of mRNA profile in cisplatin-resistant gastric cancer cell line SGC7901. World J Gastroenterol 2017; 23(7): 1189-1202
- URL: https://www.wjgnet.com/1007-9327/full/v23/i7/1189.htm
- DOI: https://dx.doi.org/10.3748/wjg.v23.i7.1189