Investigation of mitigating effect of colon-specific prodrugs of boswellic acid on 2,4,6-trinitrobenzene sulfonic acid-induced colitis in Wistar rats: Design, kinetics and biological evaluation

Table 3 In vitro kinetics of prodrugs in aqueous buffers, gastrointestinal tract homogenates and fecal matter

Prodrug	Incubation medium
	HCl buffer (pH 1.2) and stomach homogenates	Phosphate buffer (pH 7.4) and intestinal homogenates	Colon homogenate				Fecal matter
			K ± SD (min-1)1	t1/2 (min)	Prodrug hydrolyzed	BBA released	K ± SD (min-1)1	t1/2 (min)	Prodrug hydrolyzed	BBA released
BH	Stable	Negligible	0.0013	514.1	78.10%	73.60%	0.0009	723.1	62.29%	57.37%
		hydrolysis
BP	Stable	Negligible	0.0014	488.3	75.08%	71.79%	0.0010	636.7	57.38%	56.67%
		hydrolysis
BT	Stable	Negligible	0.0018	379.4	86.67%	86.39%	0.0010	662.5	69.75%	72.52%
		hydrolysis
BTY	Stable	Negligible	0.0014	484.4	70.33%	68.57%	0.0010	677.6	55.95%	55.13%
		hydrolysis

¹Average of three readings; follows first-order kinetics.