Melatonin, a novel selective ATF-6 inhibitor, induces human hepatoma cell apoptosis through COX-2 downregulation

Figure 3 Selection of the most effective sequences of each unfolded protein response pathways siRNA (A-C). All of the candidate sequences of the three unfolded protein response (UPR) pathways were examined by RNA interference, and the expression of UPR proteins was evaluated by western blotting to select the most effective one to interfere with the UPR pathway. ATF-6: Activating transcription factor 6; NC: Negative control; IRE: Inositol-requiring enzyme; PERK: Protein kinase RNA-like endoplasmic reticulum kinases.