Copyright
©The Author(s) 2017.
World J Gastroenterol. Feb 14, 2017; 23(6): 1010-1017
Published online Feb 14, 2017. doi: 10.3748/wjg.v23.i6.1010
Published online Feb 14, 2017. doi: 10.3748/wjg.v23.i6.1010
Table 1 Reverse transcription polymerase chain reaction primers
Gene name | Primer sequence | Product length, bp |
Fas | forward: 5′-GAATGCAAGGGACTGATAGC-3′ | 414 |
Reverse: 5′-TGGTTCGTGTGCAAGGCTC-3′ | ||
FasL | forward: 5′-GGAATGGGAAGACACATATGGAACTGC-3′ | 239 |
Reverse: 5′-CATATCTGGCCAGTAGTGCAGTAATT-3′ | ||
Caspase-3 | forward: 5′-GCTATTGTGAGGCGGTTGT-3′ | 270 |
Reverse: 5′-CGTTTGGAGTCCCTTTGT-3′ | ||
Caspase-8 | forward: 5′-TCTGGAGCATCTGCTGTCTG-3′ | 427 |
Reverse: 5′-TTGACGTCTGTGGTCCGTCC-3′ | ||
p53 | forward: 5′-CTTTCAACTCTGTCTCCTTC-3′ | 180 |
Reverse: 5′-TGGGCAACCAGCCCTGTCGT-3′ | ||
Bcl-2 | forward: 5′-CAGCTGCACCTGACGCCCTT-3′ | 231 |
Reverse: 5′-GCCTCCGTTATCCTGGATCC-3′ | ||
β-actin | forward: 5′-ACACTGTGCCCATCTACGAGG-3′ | 621 |
Reverse: 5′-AGGGGCCGGACTCGTCATACT-3′ |
- Citation: Mo XQ, Wei HY, Huang GR, Xu LY, Chen YL, Qi J, Xian W, Qin YC, Wei LD, Zhao LJ, Huang YQ, Xing W, Pu HQ, Wei PY, Li CG, Liang QC. Molecular mechanisms of apoptosis in hepatocellular carcinoma cells induced by ethanol extracts of Solanum lyratum Thumb through the mitochondrial pathway. World J Gastroenterol 2017; 23(6): 1010-1017
- URL: https://www.wjgnet.com/1007-9327/full/v23/i6/1010.htm
- DOI: https://dx.doi.org/10.3748/wjg.v23.i6.1010