Dendritic cells engineered to secrete anti-DcR3 antibody augment cytotoxic T lymphocyte response against pancreatic cancer in vitro

Figure 1 Cells obtained represented typical morphological characteristic of primary tumor cells and mature dendritic cells. Cells were derived from five pancreatic cancer (PC) patients and with similar shape of target cells. A: Most of the cultured primary tumor cells (over 90%) showed a DcR3-positive expression (magnification × 200); B: After cultured for 7 d, matured dendritic cells (DCs) were transfected without RNA and assembled into non-cohesive colonies, and the ablated cell showed a distinctive villiform process (magnification × 200). DCs transfected with total tumor RNA alone (C) or together with anti-DcR3 mAb mRNA (D) showed similar cell morphology to DCs transfected without RNA (C: magnification × 400; D: scanning electron microscope, bar represents 10 μm).