Influence of TBX21 T-1993C variant on autoimmune hepatitis development by Yin-Yang 1 binding

Figure 1 Identification of transcription factor binding to the T-1993C SNP site of TBX21 promoter in vitro and in vivo. A, B: EMSA analysis with biotin-labeled probes carrying the -1993T and -1993C allele and with nuclear extract from Jurkat cells and Raji cells was performed in the presence of anti-YY1 antibody; C: EMSA with biotin-labeled probes and with nuclear extract from Raji cells was performed in the presence of 100-200-fold excess of unlabeled self-oligonucleotide or YY1 probe; D: In vivo binding of YY1 to the T-1993C SNP site of the TBX21 promoter. ChIP assays with an anti-YY1, anti-C/EBPβ, ant-C-Jun, or control antibody (rabbit IgG) were performed on Jurkat cells or Raji cells. Input DNA or Immunoprecipitated DNA was used as template for PCR amplification of a 137-bp amplicon encompassing TBX21-1993. EMSA: Electrophoretic mobility shift assay; ChIP: Chromatin immunoprecipitation.