Mechanisms of autophagy activation in endothelial cell and their targeting during normothermic machine liver perfusion

Figure 3 Autophagy and liver viability following normothermic machine liver perfusion. Immunohistochemical analysis was performed for the autophagy protein LC3B in liver tissue prior to normothermic machine liver perfusion (NMP-L) and after 6 h of NMP-L. Livers deemed viable after NMP-L demonstrated LC3B immunostaining in the hepatic sinusoids prior to commencing NMP-L and at the end of perfusion. Donor livers not fulfilling viability criteria demonstrated no LC3B Immunostaining.