Analysis of 12 variants in the development of gastric and colorectal cancers

Table 1 Technical characteristics of the studied markers

Gene	ID	Type	Length, bp	Primers	Amplicon, bp
CASP8	rs3834129	INDEL	6	F-5’CTCTTCAATGCTTCCTTGAGGT3’	249-255
				R-5’CTGCATGCCAGGAGCTAAGTAT3’
CYP2E1	-	INDEL	96	F-5’TGTCCCAATACAGTCACCTCTTT3’	303-399
				R-5’GGCTTTTATTTGTTTTGCATCTG3’
CYP19A1	rs11575899	INDEL	3	F-5’TGCATGAGAAAGGCATCATATT3’	122-125
				R-5’AAAAGGCACATTCATAGACAAAAA3’
IL1A	rs3783553	INDEL	4	F-5’TGGTCCAAGTTGTGCTTATCC3’	230-234
				R-5’ACAGTGGTCTCATGGTTGTCA3’
IL4	rs79071878	VNTR	70	F-5’AGGGTCAGTCTGGCTACTGTGT3’	147/217/287
				R-5’CAAATCTGTTCACCTCAACTGC3’
MDM2	rs3730485	INDEL	40	F-5’GGAAGTTTCCTTTCTGGTAGGC3’	192-232
				R-5’TTTGATGCGGTCTCATAAATTG3’
NFKB1	rs28362491	INDEL	4	F-5’TATGGACCGCATGACTCTATCA3’	366-370
				R-5’GGCTCTGGCATCCTAGCAG3’
PAR1	rs11267092	INDEL	13	F-5’AAAACTGAACTTTGCCGGTGT3’	265-277
				R-5’GGGCCTAGAAGTCCAAATGAG3’
TP53	rs17878362	INDEL	16	F-5’GGGACTGACTTTCTGCTCTTGT3’	148-164
				R-5’GGGACTGTAGATGGGTGAAAAG3’
TYMS	rs16430	INDEL	6	F-5’ATCCAAACCAGAATACAGCACA3’	213-219
				R-5’CTCAAATCTGAGGGAGCTGAGT3’
UGT1A1	rs8175347	VNTR	2	F-5’CTCTGAAAGTGAACTCCCTGCT3’	133/135/137/139
				R-5’AGAGGTTCGCCCTCTCCTAT3’
XRCC1	rs3213239	INDEL	4	F-5’GAACCAGAATCCAAAAGTGACC3’	243-247
				R-5’AGGGGAAGAGAGAGAAGGAGAG3’

F: Forward; INDEL: Insertion/deletion; R: Reverse; VNTR: Variable number tandem repeat.