Fructo-oligosaccharide intensifies visceral hypersensitivity and intestinal inflammation in a stress-induced irritable bowel syndrome mouse model

Figure 1 Effect of water avoidance stress on rate of weight gain and visceral sensitivity. A: Rate of weight gain (g) was lower in the water avoidance stress (WAS) group (n = 13) compared to the sham-WAS (n = 14) group. Values represent mean weight gain ± SD, repeated analysis of variance (ANOVA); B: Abdominal withdrawal reflex (AWR) scores in response to colorectal distension were increased in the WAS group compared to the sham-WAS group. Lines within the box represent the median value, ends of the box represent 25^th and 75^th percentiles, and the error bars represent 5^th and 95^th percentiles. Wilcoxon two-sample test; C: Pain threshold was decreased in the WAS group compared to the sham-WAS group. Values represent mean ± SD, Student’s t-test; D: Volume threshold was decreased in the WAS group compared to the sham-WAS group. Values represent means values ± SD, Student’s t-test. ^aP < 0.05, Sham vs WAS.