Gene mutations in stool from gastric and colorectal neoplasia patients by next-generation sequencing

Table 2 COSMIC and novel mutations detected by Ion Torrent sequencing

Histopathology	Cases ID	Age, yr	Sex	TNM staging	Gene	Mutation type	COSMIC No.
Stomach
AC (D)	3	66	F	T2N0	SMARCB1	p.T72K	Novel
AC (D)	14	80	F	T4aNxM1	APC	p.D1570N	Novel
					CDH1	p.V82A	Novel
					HRAS	p.V44M	Novel
					NRAS	p.K135R	Novel
AC (D)	39	65	F	T1aN0M0	EGFR	p.A750T	COSM1651572
					DDR2	p.E523K	Novel
AC (D)	43	43	M	T4bNxM1	PTEN	p.E256G	Novel
AC (D)	77	78	M	TxNxM1	APC	p.A1582P	COSM4170230
AC (I)	100	69	M	T3N3M1	APC	p.A1582P	COSM4170230
NET	11	67	M	5% PR	CDKN2A	p.V126I	COSM13778
Dysplasia	78	77	M	T0N0M0	APC	p.A1582P	COSM4170230
Colorectal
AC	20	70	M	T3N0M0	KIT	p.N564S	COSM30732
AC	12	71	F	T3N1bM0	APC	p.A1582P	COSM4170230
					TP53	p.P72A	COSM3738520
AC	21	79	M	T2N0	APC	p.A1582P	COSM4170230
AC	22	64	F	T2N0	STK11	p.F354L	COSM21360, COSM4169323
					SMO	p.N202S	COSM5979442
AC	23	53	M	TxNxM1	APC	p.A1582P	COSM4170230
AC	31	64	M	T2N0	TP53	p.Y205D	COSM43844
AC	55	69	M	T3N0M0	KRAS	p.G12V	COSM520
					TP53	p.T172fs	COSM44371
AC	68	63	M	T3N0M0	TP53	p.Y163H	COSM43846
AC	28	77	F	T1N0	KRAS	p.G12D	COSM521
					AKT1	p.E17K	COSM34142, COSM33765
Adenoma	24	81	M		APC	p.E1295*	COSM18961
Adenoma	19	64	F		NRAS	p.Q61R	COSM584, COSM28048
					FBXW7	p.R505C	COSM22975, COSM33844
					APC	p.S1465fs	COSM18873, COSM19688 COSM19332, COSM18931, COSM13864
					ERBB2	p.V842I	COSM14065, COSM1666633
					SMARCB1	p.R377C	COSM3972885
Leiomyoma	94	69	F		TP53	p.R306*	COSM10663, COSM145026
Healthy controls
	C 2	F	27		ALK	p.L1190P	Novel
	C 8	F	33		STK11	p.Y36H	Novel

AC: Adenocarcinoma; D: Diffuse type; I: Intestinal type; NET: Neuroendocrine tumor; PR: Proliferative rate.