Pretransplantation fetal-maternal microchimerism in pediatric liver transplantation from mother

Figure 2 Detection of pretransplantation HLA-DRB1 microchimerism to non-inherited maternal antigen in pediatric recipients of liver transplants, using nested PCR-SSCP method. A: MC to HLA-DRB1 NIMA (DRB1*08:03) is positive in the child; B: MC to HLA-DRB1 NIMA (DRB1*15:01) is negative in the child. SSCP analyses were performed using nested PCR products in the recipients (children), non-nested PCR products in their mothers, and reference DNA. The SSCP band pattern of DNA of the recipients is compared with that of their mothers (arrows) and corresponding reference DNA (arrows), run in the same gel. C: Child; HLA: Human leukocyte antigen; M: Mother; SSCP: Single-strand conformation polymorphism.