Intestinal parameters of oxidative imbalance in celiac adults with extraintestinal manifestations

Table 3 Human oligonucleotide primers for detection of mRNA by RT-PCR

Gene	Primer sequence	t	PCR product
IL-1β	Forward 5’-ACA TCA GCA CCT CTC AAG -3’,	60 °C	141 bp
	Reverse 5’-AGT CCA CAT TCA GCA CAG -3’
TNF-α	Forward 5’-GCC CAG GCA GTC AGA TCA TCT TC -3’,	58 °C	181 bp
	Reverse 5-TGA GGT ACA GGC CCT CTG ATG G-3’
IL-10	Forward 5’-AGC TAT CCC AGA GCC CCA GAT CCG ATT TTG G-3’,	60 °C	328 bp
	Reverse 5’-AAG CTG AGA ACC AAG ACC CAG ACA TCA AGG CG-3’
HSP-70	Forward: 5’-GCC CCA ACA GAT TGT TGT CTT-3’,	59.5 °C	111 bp
	Reverse: 5’-CCA CCA AGC AGA CGC AGA T-3’
HIF-1α	Forward 5’-GGT TCT CAC AGA TGA TGG TG-3’,	60 °C	239 bp
	Reverse 5’-TTC TTC CTC GGC TAG TTA GG-3’
SOD	Forward: 5’-GAA GGT GGG AAG CAT TA-3’,	57 °C	300 bp
	Reverse: 5’-ACC TTT GCC CAA GTC ATC TG-3’
BAX	Forward 5’-CGT CCA ACC CAC CCT GGT CT-3’,	55 °C	195 bp
	Reverse 5’-TGG CAG CTG ACA TGT TTT CTG AC-3’
β-actin	Forward 5’-GGG TAC ATG GTG GTG CCG-3’,	54 °C	307 bp
	Reverse 5’-AGC GGG AAA TCG TGC GTG-3’

t: Annealing temperature; IL-1β: Interleukin 1β; TNF-α: Tumor necrosis factor alfa; IL-10: Interleukin 10; HSP-70: Heat-shock protein 70; HIF-1α: Hypoxia-inducible factor 1α; SOD: Superoxide dismutase.