Palmitate induces fat accumulation by activating C/EBPβ-mediated G0S2 expression in HepG2 cells

Figure 3 The effects of C/EBPβ knockdown on palmitate-induced PPARγ and its target gene expression in HepG2 cells. A: HepG2 cells were transfected with control siRNA or C/EBPβ siRNA, and C/EBPβ expression was measured by Western blotting. At least three independent experiments were conducted. Data are presented as means ± SE. ^dP < 0.01 vs control siRNA. B: C/EBPβ knockdown decreased palmitate-induced mRNA expression of PPARγ and its target genes (G0S2, GPR81, GPR109A, and Adipoq). mRNA was measured by qPCR. At least three independent experiments were conducted for each measurement. Data are presented as means ± SE. ^cP < 0.05 and ^dP < 0.01 vs control siRNA. C: C/EBPβ knockdown decreased palmitate-induced protein expression of PPARγ and G0S2. Protein was examined by Western blotting. At least three independent experiments were conducted for each measurement. Data are presented as means ± SE. ^dP < 0.01 vs control siRNA.