Prostaglandin E1 protects hepatocytes against endoplasmic reticulum stress-induced apoptosis via protein kinase A-dependent induction of glucose-regulated protein 78 expression

Figure 1 TG induced endoplasmic reticulum stress in L02 cells. L02 cells were treated with 1 μmol/L TG for 6, 12, 24, 36 and 48 h. Expressions of p-PERK, p-eIF2α, GADD34, GRP78, sXBP1 and CHOP were assessed by western blotting. Representative blots from three independent experiments are presented. The results of densitometric analysis are presented as a fold-change compared to that at 0 h (^aP < 0.05; ^bP < 0.01). CHOP: C/EBP homologous protein; ER: Endoplasmic reticulum; GADD34: Growth arrest and DNA damage-inducible gene34; GRP78: Glucose-regulated protein 78; p-eIF2α: Phospho-eukaryotic translation initiation factor-2α; p-PERK: Phospho-PKR-like ER kinase; sXBP1: Spliced X box-binding protein1; TG: Thapsigargin.