Copyright
©The Author(s) 2017.
World J Gastroenterol. Oct 28, 2017; 23(40): 7242-7252
Published online Oct 28, 2017. doi: 10.3748/wjg.v23.i40.7242
Published online Oct 28, 2017. doi: 10.3748/wjg.v23.i40.7242
Table 2 Primer sequences used for real time-polymerase chain reaction
| Gene | Forward primer | Reverse primer | Cycles | Temperature, °C |
| IL-6 | 5’-GAAGTAGGGAAGGCCGTGG-3’ | 5’-CTCTGCAAGAGACTTCCATCCAGT-3’ | 40 | 60 |
| MCP-1 | 5’-GTTGGCCTCAGCCAGATGCA-3’ | 5’-AGCGTACTCATTGGGATCATCTTG-3’ | 40 | 60 |
| MIP-1α | 5’-TGCCCTTGCTHTTCTTCTCTGCACCATGGC-3’ | 5’-GGCAAATTCCACGAAAATTCATTGCTGACT-3’ | 40 | 60 |
| β-actin | 5’-CATCCTGCGTCTGGACCT-3’ | 5’-TCAGGAGGAGCAATGATCTTG-3’ | 40 | 60 |
- Citation: Duan LF, Xu XF, Zhu LJ, Liu F, Zhang XQ, Wu N, Fan JW, Xin JQ, Zhang H. Dachaihu decoction ameliorates pancreatic fibrosis by inhibiting macrophage infiltration in chronic pancreatitis. World J Gastroenterol 2017; 23(40): 7242-7252
- URL: https://www.wjgnet.com/1007-9327/full/v23/i40/7242.htm
- DOI: https://dx.doi.org/10.3748/wjg.v23.i40.7242