Mutational analysis of hepatitis E virus ORF1 "Y-domain": Effects on RNA replication and virion infectivity

Figure 1 Schematic representation of hepatitis E virus nonstructural polyprotein (ORF1) domain organization, showing the undefined Y-domain. Saturation mutations covering the entire Y-domain (nts 650-1339; 10 constructs of 68 bases each) as well as specific amino acid (C₃₃₆, C₃₃₇ and W₄₁₃) mutations within the predicted membrane-binding motif are shown. MTase: Methyltransferase; Y: Undefined; PCP: Papin-like cysteine protease; P/HVR: Proline-rich/hypervariable region; X: Macro; Hel/NTPase: Helicase/nucleotide triphosphatase; RdRp: RNA-dependent RNA polymerase.